Lereclus for the gift of the pIC333 plasmid. This work was supported by Agence Nationale de la Recherche (ANR) (France) under the ANR-05-PNRA-013 B. cereus contract. “
“In this multidisciplinary study, we combined morphological, physiological, and phylogenetic
approaches to identify three dominant water bloom-forming Cyanobacteria in a tropical marine mangrove in Guadeloupe (French West Indies). Phylogenetic analysis based on 16S rRNA selleck screening library gene sequences place these marine Cyanobacteria in the genera Oscillatoria (Oscillatoria sp. clone gwada, strain OG) or Planktothricoides (‘Candidatus Planktothricoides niger’ strain OB and ‘Candidatus Planktothricoides rosea’ strain OP; both provisionally novel species within the genus Planktothricoides). Bioassays showed that ‘Candidatus Planktothricoides niger’ and ‘Candidatus Planktothricoides rosea’ are toxin-producing organisms. This is the first report of the characterization of Cyanobacteria colonizing periphyton mats of a tropical marine mangrove. We describe two novel benthic marine species and provide new insight into Oscillatoriaceae and their potential role in marine sulfide-rich environments such as mangroves. “
“In order to evaluate the biochemical
characteristics of 14 substrains of Mycobacterium bovis bacillus Calmette Guérin (BCG) – Russia, Vincristine Moreau, Japan, Sweden, Birkhaug, Danish, Glaxo, Mexico, Tice, Connaught, Montreal, Phipps, Australia
and Pasteur – we performed eight different biochemical tests, including those for nitrate reduction, catalase, niacin accumulation, urease, Tween 80 hydrolysis, pyrazinamidase, p-amino salicylate degradation and resistance to thiophene 2-carboxylic acid hydrazide. Catalase activities of the substrains were all low. Data for nitrate reduction, niacin accumulation, Tween 80 hydrolysis, susceptibility to hydrogen peroxide and nitrate, and optimal pH for growth were all variable among these substrains. These findings suggest that the heterogeneities of biochemical Y-27632 order characteristics are relevant to the differences in resistance of BCG substrains to environmental stress. The study also contributes to the re-evaluation of BCG substrains for use as vaccines. Biochemical tests are currently used as a technique for the identification of bacterial species. Recently, several studies have investigated the physiological meaning of the biochemical characters in the genus Mycobacterium. Sohaskey and colleagues reported variable nitrate production among Mycobacterium bovis bacillus Calmette Guérin (BCG) substrains in relation to survival in host cells (Sohaskey, 2008; Sohaskey & Modesti, 2009). Recycling of NAD and NAD-quinoline reductase relevant to the latent infection of Mycobacterium tuberculosis and resistance to oxidative stress, respectively, have also been reported (Boshoff et al., 2008).
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