Similarly, exosomes expressing TGFβ derived from the malignant effusion of cancer patients
were reported to promote the increase in number and functionality of Treg in vitro [62]. Another evidence has been reported by Clayton et al., who showed that Autophagy inhibitor exosomes isolated from different tumor cell lines carry surface TGFβ and inhibit T cell proliferation by skewing IL-2 responsiveness in favor of Treg and away from cytotoxic cells [63]. It is worth mentioning that TGFβ-expressing exosomes can also be involved in physiological immune homeostasis. In fact, a recent study indicates that TGFβ expressed in thymic exosomes is required for the generation of Foxp3+ Treg in peripheral tissues, such as lung and liver, and participate in the maintenance of physiological immune
tolerance [64]. The role of tumor exosomes in promoting the expansion of immunoregulatory cell components are beginning to be investigated also in in vivo murine models, representing a crucial step for proving a true involvement of this pathway in immunosuppression and tumor progression. In this regard it should be pointed out that one major hurdle of this type of studies has been so far to assess pharmacokinetics of the injected exosomes that, due to their small dimension, might behave differently compared to whole cells. Technical advances of the PLX3397 last years have enabled the investigating groups not only to trace exosomes after in vivo administration PD184352 (CI-1040) but also to analyze the interaction pathways with host cells, an issue that is still poorly investigated. Most of the experimental evidences on the immunosuppressive role of tumor exosomes point to a potential involvement in the expansion of MDSC, while less information about
the impact of these organelles on Treg, once injected in vivo, are presently available. Immune suppressive pathways generated by adoptively transferred tumor exosomes have been observed in the TS/A mammary tumor murine model, where injected nanovesicles were found to interact with CD11b+ myeloid precursors in the bone marrow (BM) and to block BMDC differentiation by inducing IL-6 production and Stat3 phosphorylation [65]. Similarly, in a breast carcinoma model, tumor-derived exosomes were demonstrated to skew BMDC differentiation toward an MDSC phenotype promoting tumor progression, through a prostaglandin E2 and TGFβ-mediated pathway [66]. Recent data also demonstrated a pivotal role for MyD88 in tumor exosome-mediated expansion of MDSCs and promotion of lung metastasis in C57BL/6j (B6) mice [67]. Likewise, Chalmin et al.
No related posts.