Rat colitis model (group B, C and D) was established by intrarectal administration with 100 mg/kg body weight of TNBS (in 0.25 ml 50% ethanol), group A was administered the same dose of 0.9% sodium chloride solution in 0.25 ml 50% ethanol using the same technique. Then group C was treated with 100 mg/kg body of curcumin and the same dose of SASP for group D by intragastric administration daily. Rats in group A and B were respectively treated with 100 mg/kg body weight of 0.9% sodium chloride solution. Colon intestinal

and serum sample were collected after 8 days. Evaluated p38 MAPK assay weight loss, stool consistency, and blood in feces in each group. The disease activity index (DAI) was calculated by assigning well-established and validated scores for parameters. Evaluated colonic mucosa damage index (CMDI) and histological activity index (HAI). Determined IL-17 and IL-23 levels with immunohistochemistry in colonic mucosa, and with enzyme-linked immunosorbent assay (ELISA) in serum. Results: Compared

with the TNBS-induced colitis group, the scores of DAI, CMDI, HAI, the expression of IL-17, IL-23 in colonic mucosa and serum were all decreased in the curcumin-treated group (P < 0.05). While there was no significant difference in DAI, CMDI, HAI, expression of IL-17, IL-23 Daporinad between the curcumin-treated group and SASP-treated group (P > 0.05). Conclusion: Curcumin can attenuates inflammation through inhibition of IL-17 and IL-23 in experimental rats colitis. Key Word(s): 1. curcumin; 2. colitis; 3. IL-17; 4. IL-23; Presenting Author: ZHANG JING Additional Authors: LIBI MIN Corresponding Author: LIBI MIN Affiliations: First affiliated hospital of nanchang university Objective: To explore the effect of NF-κB Decoy ODN enema on the BALB/C mice with chronic intestinal fibrosis induced by TNBS/50% EtOH, and

research the best treatment of NF-κB Decoy ODN to intestinal fibrosis. To observe the experimental mice disease activity see more index (DAI), analyze the change of histological pathological, find the hyperplasia degree of collagen fibers, research the expression of IL-1β, TNF-α, Col-III mRNA and NF-κB, TGF-β1 protein. So that we can study the mechanism of NF-κB Decoy ODN on the BALB/C mice with chronic intestinal fibrosis induced by TNBS, and provide the experimental basis for IBD treatment. Methods: 1. Establishment of IBD mice models BALB/C mice are fasting but free for water for 24 hours, ether anesthesia, use 3.5 F catheter insert into the intestine from anus and the depth is about 5.5 cm. Give 2 mg TNBS/50% EtOH 100 ul enema, and inverse the mice for 60 seconds. Last for 6 weeks, once for a week, all groups of mice get executed one week after the last enema. 2.

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