Feeding and Supplementation Protocols Animals were fed ad libitum standard chow (Labina, Ralston Purina do Brasil®) and water. CR supplementation or placebo (water) was administered via gavage. The researchers were blinded to the treatments. Supplementation protocol consisted of two daily dosages of 300 mg each, for 5 days. We had previously found this protocol to be effective in increasing total CR content by approximately 15% in Wistar rats’ gastrocnemius ALK tumor muscle (unpublished data). Moreover, the total amount of CR administered in our supplementation
protocol is equal to or even more than those amounts used in other studies that also have shown increased total CR at around 25% [17, 18]. Experimental Procedure All animals
underwent a 12 h overnight fasting period before the experimental protocol. The animals were weighed immediately prior to exercise, and then the workload utilized during the experimental protocol was determined, accounting for changes in BW. The animals were then submitted to GW-572016 clinical trial intermittent high-intensity check details swimming exercise bouts of 30-second duration. The bouts were performed using a 50% higher external load (attached to the rat’s chest) than the one correspondent to the anaerobic threshold. Swimming bouts were interspersed by 2-minute rest intervals. Animals were submitted to as many bouts as possible until fatigue. Fatigue was determined when the rat was submerged for longer than 3 seconds. Experiment 2 Once it was demonstrated that the proposed CR supplementation protocol had effectively improved time-to-exhaustion in an intermittent high intensity exercise, a second experiment was carried out in order to evaluate whether CR supplementation was able to influence glycogen content and blood
lactate concentration in a sub-maximal (fixed number of bouts) intermittent high intensity exercise protocol. Animals Twenty eight male Wistar rats, weighing 217.55 ± 3.54 g were kept on the same conditions as previously described for experiment 1. The procedures for randomization 3-oxoacyl-(acyl-carrier-protein) reductase and group assignment (CR – n = 14; Pl – n = 14), the anaerobic threshold test, feeding and supplementation protocols were also identical to those of experiment 1. Experimental Procedure All animals underwent a 12 h overnight fasting period before the experimental protocol. They were submitted to 6 bouts of 30-second swimming exercise with supra anaerobic threshold workloads (50% higher than the anaerobic threshold correspondent load). Immediately before testing, animals were weighed and workloads were then calculated. Swimming bouts were interspersed by two-minute rest intervals. Blood and Tissue Collection Blood samples (25 μl) were drawn from the tail vein at rest, after a ten-minute unloaded warm-up, and at the end of the two-minute recovery period correspondent to each of the 6 swimming bouts.
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