ATPase6 helps to maintain the mt genome integrity, and mutations in ATPase6 are associated with overproduction of reactive oxygen species

(ROS) in a variety of diseases; however, its role in POI has not been evaluated. Therefore, we planned to evaluate the potential role of ATPase6 gene mutations and associated oxidative stress in idiopathic cases of POI.

This pilot study included: 20 cases of POI with FSH level of > 40 mIU/ml; 4 cases of occult ovarian insufficiency (occult OI) with irregular menses and mean FSH levels of 16.4 mIU/ml; and 20 age-matched healthy female controls (FSH 2-5 mIU/ml). ROS levels in blood plasma were measured Alvespimycin in vivo by luminol-dependent chemiluminescence assay Buparlisib concentration and the ROS values were expressed as relative light unit per minute (RLU/min). mtDNA ATPase6 gene was amplified and sequenced from the blood lymphocyte DNA.

Of all, 50% patients showed nucleotide changes in the ATPase6 gene, as compared to 10% in controls, and the majority of these mutations were non-synonymous. ATPase6 mt.8684 C > T and mt.9094 C > T were found to be significantly (P < 0.005) higher in cases as compared to controls. ROS levels were found to be significantly (P < 0.005) higher in POI and occult OI patients compared to controls and nucleotide changes were found to positively correlate with ROS levels. Moreover, ROS production was found to positively correlate (r = 0.7038,

P < 0.001) with FSH levels of the patients (POI and OI) compared to controls.

This pilot study clearly demonstrates for the first time ATPase6 gene nucleotide alterations and elevated ROS levels in idiopathic cases of POI. Therefore, it may be possible that OS associated with Dibutyryl-cAMP ic50 ATPase6 gene

mutation may be causal in idiopathic cases of premature OI. However, larger studies with inclusion of more cases of both POI and occult OI are required to strongly establish the correlation between oxidative stress and mitochondrial nucleotide alterations in the pathogenesis of POI. Such cases with OS-induced POI may benefit immensely by early diagnosis and prompt antioxidant administration.”
“The optical constants n and k of holmium (Ho) films were obtained in the 3-1340-eV range from transmittance measurements performed at room temperature. Thin films of Ho with various thicknesses were deposited by evaporation in ultra high vacuum conditions and their transmittance was measured in situ. Ho films were deposited onto thin C-film substrates supported on high transmittance grids. Transmittance measurements were used to obtain the extinction coefficient k of Ho films. The refractive index n of Ho was calculated with Kramers-Kronig analysis; in order to do this, k data were extrapolated both on the high and on the low energy parts of the spectrum by using experimental and calculated k values available in the literature.

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