The CFP and the filters of the cigarettes were extracted separately with isopropanol (99.9%
purity from Fluka) and analysed by GC/MS. After passing through the filter and the CFP, the smoke was collected in a Tedlar bag and appears throughout the text as “gas fraction”. According to the ISO 4387, total particulate matter (TPM) and nicotine (N) refers to that collected in the CFP traps. In this work, in order to properly evaluate the additives effect, the particulate matter condensed in the filters of the cigarettes has also been quantified and analysed. Results are presented as TPM for the particulate matter condensed in the CFP traps and TPM(F + T) which IDH inhibitor indicates the total amount of TPM contained in the smoke, i.e., that condensed in the filters of the cigarettes plus that condensed in the CFP traps located
after the filters. TPM(F + T) is not commonly reported since it is partially retained in the filters, but it is interesting to analyse it to better evaluate the effect of the additive. Nicotine and other components of the particulate matter are also presented maintaining the same nomenclature; N(F + T), corresponds to the amount of nicotine collected in the filters of the cigarettes plus that in the traps. The weight of tobacco smoked (WTS) was calculated as the difference between the weight of tobacco per cigarette (WTC) before and after Small Molecule Compound Library smoking. The amount ASH corresponds to the total mass of ash collected and expressed on an additive free basis (taking into account the WTS, the initial WTC and the weight fraction of additive per cigarette). In this work 80 compounds are reported in the case of
the TPM and 32 in the case of the gas fraction. The analytical procedure was explained elsewhere [22]. As explained there in, response factors for nicotine in the TPM and CO, 1,3-butadiene, HCN, isoprene, acrolein, propionaldehyde, crotonaldehyde, benzene, toluene and acetaldehyde in the gas fraction were obtained. Consequently, results are semi-quantitative. Standard deviations in the three replicate runs lower than 25% for all the compounds analysed were obtained. The results of the analysis of the gas fraction by FID for one of the brands Amoxicillin are shown in Table 4, while those of the particulate matter carried out by GC/MS are in Table 5. The sum of all the compounds identified and quantified in the gas fraction by FID has been named as TG (in Table 3) and that of the compounds from the TPM(F + T) analysed by GC/MS appears in the following as Liq(F + T) (Table 3). Table 3 shows the results obtained for the average mass fraction of additive loaded (CL), the WTS, TPM(F + T), TPM, N(F + T), Liq(F + T), TG, and ASH, for the ten commercial brands when no additive was used, and when the three additives were included. The average (Av), minimum (min) and maximum (Max) values of the variables for each set of experiments has also been included in order to facilitate comparisons.
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