βg   and βs   are corrections to the overall mean and measure consistent

differences between genders and smoking status. The random effects wi   are assumed to be normally distributed Selleckchem Vemurafenib with a mean of zero and standard deviation σ12, with σ12 quantifying the inter-individual variability. The term ϵij   represents the residual errors which are assumed to be normally distributed (on the log-scale) with mean zero and standard deviation σ22, with σ22 quantifying intra-individual variability. The models were fitted using Markov Chain Monte Carlo (MCMC) methods in WinBUGs (Lunn et al., 2000), within a Bayesian framework. For elements where a large proportion of measurements fall below the LOQ, the mixed effects modelling may result in biased estimates of the fixed effects and variability. Although there is no standard cut-off point, the decision was thus made to limit the mixed effects analysis to only those elements where no more than one third of measurements fall below the LOQ to minimise the bias arising from censored data. All urine samples were analysed by each of the six ICP–MS methods and the summarised results are presented

in Table 3. Each method used different quality control approaches and these are summarised below. All elements determined Dolutegravir order in the CRMs were found to be within the acceptable range for each analyte. The CRMs used, the ranges and results are presented in Table 2. Generally the standard deviations of the analytes in CRM samples were less than 10%. Successful participation in external quality assurance schemes was obtained

for all 18 elements for which the schemes were available. The schemes are stated for each of these elements in Table 2. Analyte concentrations of the rarer elements in internally prepared QC materials showed variation in recoveries. For the elements that were analysed with hydrochloric Interleukin-2 receptor acid diluent (Method 4) the recoveries varied in the prepared frozen spiked pool samples, with low values for silver (56% for 50 ng/L spike and 66% for 200 ng/L spike) to good spiked recoveries for osmium (103.3% for 50 ng/L spike and 103.9% for 200 ng/L spike). For rare elements diluted with nitric acid (Method 5) recoveries ranged from 75.4% for gold and 120.8% recovery for hafnium. In addition, these elements were also analysed with samples containing daily prepared spikes, which gave an over recovery for gold of 125.2 and 103.1% for hafnium. It should be noted that no storage or stability tests had been undertaken on the in-house frozen pool samples and it is likely that both the silver and gold were not stable throughout the freeze/thaw process. The standard 2.5 μg/L check analysed throughout the silver and gold analysis showed good stability and accuracy.

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