02 – - Brevundimonas diminuta c 0.01 – - Corynebacterium accolens 0.01 0.003 0.002 Corynebacterium durum 0.01 0.152 0.775 Corynebacterium matruchotii 0.07 0.192 8.934 www.selleckchem.com/products/ulixertinib-bvd-523-vrt752271.html Corynebacterium tuberculostearicum 0.01
0 0.009 Enterobacter cancerogenus c 0.01 – - Enterococcus faecalis c 0.04 9.04 0.02 0.01 – - Fusobacterium nucleatum 0.02 0.07 0.824 3.219 Gemella haemolysans c 0.01 – - Haemophilus parainfluenzae 0.03 3.761 3.110 Kingella denitrificans 0.01 0.103 0.304 Lactobacillus johnsonii 0.01 0.001 Neisseria subflava 0.01 4.420 0.051 Propionibacterium acnes 0.21 0.03 0.01 0.02 0.03 0.95 1.21 0.017 0.150 Rothia aeria 0.02 Palbociclib 0.208 1.048 Staphylococcus hominis 0.02 0.002 Staphylococcus saprophyticus 0.01 Staphylococcus sciuri 1.36 20.32 0.56 1.66 0.03 0.01 0.001 0.003 Streptococcus mitis c 0.01 0.01 – - Streptococcus pseudopneumoniae 0.03 4.890 2.344 Streptococcus salivarius 0.02 0.02 3.747 0.029 Streptococcus sanguinis 0.12 11.145 9.028 Treponema denticola c 0.03 0.72 0.03 0.01 – - Entospletinib molecular weight Triticum aestivum 0.02 0.001 Veillonella parvula 0.01
0.003 SUMd 1.88 30.74 0.67 2.44 0.04 0.12 1.20 1.50 32.942 29.935 aThe relative abundance (%) of bacterial species observed in
this study. Bacterial samples from the tongue, palate, and incisors were pooled. bThe relative abundance (%) of bacterial species obtained from an analysis of data generated by Keijer Baricitinib et al. [6]. Saliva from 71 individuals and supragingival plaque from 98 individuals was pooled. cNot present in the study by Keijer et al. but found in the study by Paster et al. [24] dTotal contribution of bacterial species shared between mouse and humans Conclusion To our knowledge, this study presents the first successful application of the Roche/454 FLX Titanium to 16S rRNA-based microbial community analysis. Using this new method, the oral bacterial community of captive mice was found to be relatively simple, consisting mainly of a few species in the genera Streptococcus, Staphylococcus, Lactobacillus, Halomonas and Enterococcus. In addition, the mouse oral bacterial community was not affected by TLR2 deficiency. This survey provides a basis for future studies of the role of periodontal pathogens in the murine model of periodontitis. Methods Mice TLR2-deficient mice of the C57BL/6 background were kindly provided by Shizuo Akira (Osaka University, Japan) and have been bred and maintained at the Laboratory Animal Facility of our school in pathogen-free conditions for five years. Pathogen-free wild-type (WT) C57BL/6NCrljBgi mice were 6 or 8 weeks old upon purchase from the Orient Co.
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