, 1999 and Stio et al., 2002). Taken together, our results indicate a significant increase in Hsp70 serum levels with increasing degree of inflammation. We found negative correlations between Hsp70 levels and micronutrients including vitamin D, vitamin B12, as well as folate, which could be linked to the immune modulating effects of these vitamins. In order to study the disease burden of the elderly population in a low income, sub-Saharan region, a census was organized in the Ntam health area, situated in the predominantly rural southwest province of Cameroon,
followed by a systematic enrolment of all inhabitants 60 years of age or older. The study was approved by the ethical committee of the University of Yaoundé 1, Cameroon. All participants gave their informed consent. For the present sub-study, 56 women (aged between 60 and 80 years, mean Bafilomycin A1 research buy age 66.4 ± 5.4 years (±S.D.) and 81 men click here (aged between 60 and 86 years, mean age 67.2 ± 6.5 years participated. The medical histories, current medical and functional statuses of all the participants were obtained by questioning the participants and by physical examination. Most of the participants were involved in activities which resulted in daily exposure to sun for long periods. In addition, the study region was endemic for infectious and parasitic diseases which reflect the health
status of its inhabitants (Ford et al., 2007). Table 1 provides Ribose-5-phosphate isomerase details of the characteristics of the participants. Venous blood was obtained after overnight fasting. After separation from blood cells, serum was aliquoted and stored at −20 °C. Anticoagulated venous blood was used for the white blood cell (WBC) enumeration counts (using counter chambers), and for the determination of erythrocyte sedimentation rate (ESR, Westergren). CRP was quantified by immunonephelometry using the N high sensitivity CRP kit obtained from Dade Behring (Marburg GmbH, Germany). Values <4 mg/l were considered normal. The monoclonal antibody directed against Hsp70
(clone c92f3a-5, spa-810) was purchased from Stressgen (Victoria, Canada). This antibody, as reported by the manufacturer, is specific for the inducible form of Hsp70 and does not cross-react with the constitutive heat shock cognate 70 (Hsc70) or dnak from bacterial origin. Hsp70 in serum was detected as previously described (Njemini et al., 2005a). Briefly, plates were coated with the primary antibody (100 μl; 5 μg/ml) diluted in 0.1 M carbonate buffer (pH = 9.6). After overnight incubation at 4 °C, the coated plates were washed six times with phosphate buffered saline (PBS) containing 0.1% Tween-20 (PBS/T) and non-specific binding sites blocked by incubation with 300 μl of PBS/T containing 1% bovine serum albumin (BSA) (PBS/T/BSA) for 2 h at 37 °C on a shaker.
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