5 mg L−1 of WBM for 3 weeks. The same exposure caused histopathological changes in gills and changes in blood plasma in juvenile Atlantic cod. Interestingly, 1–10 mg L−1 suspensions of WBM had a positive effect on feeding efficiency, growth and survival in cod larvae after 14 days exposure. The positive effects were assumed to be from particles of a particular size stimulating Inhibitor Library solubility dmso feeding activity. Feeding efficiency and growth in blue mussel larvae were reduced after exposure to 4 mg L−1 suspensions of used barite-based WBM, whereas similar exposure to barite alone stimulated growth. Berland et al. (2006) made a field validation of the results from Bechmann et al. (2006) by exposing caged scallops and blue mussels to
an offshore discharge of WBM cuttings for 5 weeks. Scallops caged 250 m from the platform at a depth of 35 m showed increased GST enzyme activity and reduced gonad weight. DNA damage was seen in the mussels from the same cage. Filtration CT99021 solubility dmso rate was reduced in both species, but shell growth was not affected. The other
endpoints measured by Bechmann et al. (2006) were not affected (LMS, tolerance in mussel to air exposure, proteomics, and barium body burden). Exposure levels around the cages were not measured, but the average concentration of suspended cuttings where effects were found was estimated to be 0.15 mg L−1. This corresponds well with the lowest concentration of suspended cuttings eliciting effects in the laboratory studies mentioned above (0.5 mg L−1). From their experiments Bechmann et al. (2006) proposed 0.8 mg L−1 as a chronic PNEC for suspended cuttings. Smit et al. (2008) estimated PNEC values of 7.6 mg L−1 and 17.9 mg L−1 respectively for suspended bentonite and barite clays on basis of SSDs from tests with 12–15 marine species. Although these PNEC estimates were made in somewhat different ways and hence are not directly comparable, the far lower PNEC for whole WBM cuttings proposed by Bechmann et al. (2006) could indicate that there may be other effects factors
in play than just physical stress from the clay particles. The proposed PNEC is also within the typical range of natural SPM (suspended particulate matter) levels in the open NS (0.2–1 mg L−1, Eisma and Kalf, 1987) which also indicates that WBM in suspension may elicit tuclazepam stronger effects than physical stress from suspended particles. Studies on effects of suspended cuttings on sessile filter feeders such as sponges and cold water corals have not been published and there are only a few published studies on the effects of cuttings particles settling onto these organisms. Larsson and Purser (2011) found that the cold water coral Lophelia pertusa was able to survive repeated, slight smothering by natural sediment and drill cuttings, but polyp death occurred when wholly covered by the particles. The response to cuttings and natural sediment did not differ. It was concluded that the current effects level from non-toxic burial of 6.
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