Analysis was performed by FACScan flow cytometer. Results Parthenolide effectively inhibits the growth of human lung cancer cells through induction of apoptosis and cell cycle arrest It has been reported that parthenolide has antitumor effects on various cancer cells. Hence, we examined the inhibition effect of PTL on human NSCLC cells by treating the cells with various concentrations for 48 h and then conducting SRB and MTT assay. As is shown, PTL had a dose dependent growth inhibition effect on NSCLC cells Calu 1, H1792, A549, H1299, H157, and H460. To characterize the mechanism by which PTL induces growth inhibition in human NSCLC cells, we first determined the effect of PTL on induction of apoptosis by western blot analysis.
The data showed that PTL could induce cleavage of apoptotic proteins such as CASP8, CASP9, CASP3 and PARP1 both in concentration and time dependent manner in tested lung cancer cells, BAPTA-AM indicating that apoptosis was trigged after PTL exposure. In addition to induction of apoptosis, PTL also induced G0 G1 cell cycle arrest in a concentration dependent manner in A549 cells and G2 M cell cycle arrest in H1792 cells. The difference in cell cycle arrest induced in these two cell lines may be due to the p53 status. Collectively, these results show that PTL inhibits the growth of human lung cancer cells through induction of apoptosis and or cell cycle arrest. Parthenolide triggers extrinsic apoptosis by up regulation of TNFRSF10B expression In order to understand the molecular mechanism of PTL induced apoptosis in NSCLC cell lines, several apoptosis related proteins were examined.
Data showed that TNFRSF10B was up regulated after exposure to PTL. After TNFRSF10B expression was knocked down using siRNA method, the cleavage of CASP8, CASP9, CASP3 and PARP1 induced by PTL treatment CORM-3 molecular weight were receded compared with control siRNA knockdown. The analysis of Annexin V staining showed that apop tosis was inhibited when TNFRSF10B was knocked down. It can be concluded that PTL up regulates TNFRSF10B and contributes to apoptosis in duction in lung cancer cells. CFLAR is down regulated in parthenolide induced apoptosis Since CFLAR is an important modulator of extrinsic apoptotic pathway, we also detected the levels of CFLAR and found that both CFLARL and CFLARS were down regulated in a concentration and time dependent manner after PTL treatment. Compared with control cells, cleavage of pro caspases and PARP1 were weaker in A549 CFLARL cells which over expressing CFLARL. Annexin V staining showed PTL induced less apoptosis in A549 CFLARL cells than that in control cells. We got same results in H157 CFLARL cells. This implicated that CFLARL could prevent human lung cancer cells from apoptosis induced by PTL treatment.
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