cDNA-AFLP analysis For each of the 43 primer combinations, 40-100 different transcript derived fragments (TDFs), which ranged from 50 to 800 bp, were visualized as bands (Figure 2). Figure 2 Representative results of polyacrylamide learn more gel of cDNA-AFLPs generated by the primer combinations E11/MCG. Wells 1-10, 11-20, and M present non-infected, infected and 100 bp DNA size marker, respectively. Gh.821 and Gh.8221.1 represent two differentially expressed transcript derived fragments (DE-TDFs) that were identified as autophagy protein 5. Analysis of the expression profiles of
the infected and selleck chemicals noninfected samples between replicates revealed 55 differentially expressed TDFs (DE-TDFs) that showed the same pattern in all replicates. Fifty-one of these DE-TDFs were isolated and sequenced. The remaining four DE-TDFs could not be cloned and were excluded from analysis. Out of the 51 sequenced DE-TDFs, 36 showed similarity to known gene sequences in databases (Table 1), whereas 15 DE-TDFs did not show homology to any known nucleotide selleck compound or amino acid sequences. All 51 TDFs sequences were submitted to the NCBI database with accession numbers assigned and reported in Table 1. Table 1 Homologies of the transcript derived fragments (TDFs)
to known sequences in the databases. TDF Length (bp) Accession number I/R Annotation (plant, accession number) E-value Stress response/defense Gh16122 444 GT222039 I Proline-rich protein (Cladrastis kentukea, AAG15241.1) 1e-12 Gh11114 158 GT222037 R Modifier of snc1 (Ricinus communis, XP_002522998.1) 6e-04 Gh11112
157 GT222036 R Modifier of snc1 (Ricinus BCKDHB communis, XP_002522998.1) 6e-4 Gh921 191 GT222045 R Autophagy protein 5 (Glycine max, AM087008.1) 3e-19 Gh8221.1 198 GT222040 R Autophagy protein 5 [Glycine max, AM087008.1) 5e-08 Gh8221.2 190 GT222035 R Autophagy protei n (Glycine max, AM087008.1) 4e-19 Gh821 191 GT222047 R Autophagy protein 5 (Glycine ma x AM087008.1) 1e-29 Gh542 316 GT222056 I hypothetical protein with lysine domain (Medicago sativa, XP_002278178.1) 3e-22 Gh7111 69 GT222032 I Serine-rich protein-related, Cichorium intybus, TA1423_13427 7e-51 Gh16121 162 GT222038 I Serine-rich protein-related, Cichorium intybus, TA1423_13427 1e-49 Cell Metabolism Gh1574 526 GT222018 I Phosphatidyl glycerol specific phospholipase C-like (Sweet orange, EY651478.1) 1e-40 Gh511 113 GT222066 R L-asparaginase (Ricinus communis, ref-XM_002510114.1) 5e-06 Gh7123 263 GT222042 R Glycerophosphoryl diester phosphodiesterase (Ricinus communis, XP_002512887.1) 4e-27 Gh532 181 GT222058 R Retroelement pol polyprotein-like (Arabidopsis thaliana, BAB10790) 1e-14 Protein synthesis/destination Gh1633 416 GT222024 I 50 S ribosomal protein L15 (Ricinus communis, XP_002531621.1) 2e-19 Gh1631 416 GT222023 R 50 S ribosomal protein L15 (Ricinus communis, XP_002531621.1) 5e-18 Gh553-2 323 GT222065 R Ubiquitin-protein ligase (Vitis vinifera, XM_002305323.
No related posts.