It reacts with peroxy radicals 10,000-fold faster than do polyunsaturated lipids. Therefore, vitamin E is potentially useful as therapeutic www.selleckchem.com/products/Imatinib(STI571).html agent in the treatment of several disorders associated with oxidative damage.[6] It might diminish lipid peroxidation (LPO) induced by heavy metals, including dichromate and protects the body’s biological systems.[7] The first well known and the most established function of vitamin E is the regulation of reproductive functions in both male and female.[8] Because of the health problems induced by many environmental pollutants, much effort has been expended in evaluating the relative antioxidant potency of vitamin E.[9] In light of the above data, the present study was undertaken to assess the effects of chromium on ovarian steroidogenesis and its possible protection by ��-tocopherol.
MATERIALS AND METHODS Chemicals All the chemicals were of analytical grade and obtained from commercial sources. Animals Adult Wistar rats (24), aged about 60 days with average body weight of 140 �� 10 g were obtained from National Institute of Nutrition (NIN), Hyderabad. The animals kept in polypropylene cages were maintained under standard conditions prescribed by the committee for the purpose of control and supervision on experiments on animals (CPCSEA). The experimental protocol was approved by the Institutional Animal Ethics Committee (Approval No. I/7/2012). Experimental design A total of 24 rats were randomly divided into four groups with six rats in each. Group 1 was maintained as normal, while group 2 rats acted as Cr toxicity control.
These rats were given Cr as K2Cr2O7 dissolved in sterile saline (NaCl 0.9%) @ 10 mg/kg b.wt. as a single s.c. injection. Group 3 received Cr as above, but along with ��-tocopherol, daily for 14 days by oral gavage. Group 4 was maintained as ��-tocopherol control and was given ��-tocopherol daily for 14 days by oral gavage. The study was approved by Institutional Animal Ethics Committee. In this experiment, the dose of Cr to induce oxidative stress was based on a report by Biber et al.[10] The selected dose of ��-tocopherol was as per Laura et al.[7] who stated that ��-tocopherol at a dose of 125 mg/kg body wt. for 14 days effectively protected the kidney against Cr-induced alteration in lipid patterns. Body weights were recorded at the beginning and at the end of experiment.
Further, the rats were observed for occurrence of estrus cycle for 3 consecutive cycles. After completion of 14 days, the blood samples were collected from retro-orbital plexus of experimental rats for studying serum biochemical profile (ALT, BUN, creatinine and total protein). Carfilzomib Then all the rats were euthanized. Uterus along with ovaries was collected immediately and ovaries were kept in ice cold phosphate buffer. A portion of the ovaries was homogenized with tissue homogenizer individually to make 10% homogenate to assay antioxidants, peroxidation and functional markers.
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