JPG is the recipient of a Murdoch University Postgraduate Scholarship. Electronic supplementary material Additional file 1: Figure S1. ClustalW alignment of S. nodorum (A) Gba1 and (B) GgaA with fungal orthologues. Figure S2. (A) Agarose gel electrophoresis of PCR products arising from the amplification of the (A) GgaA locus of the created S. nodorum mutants. Targeted Insertion of the phleomycin cassette in place of the S. nodorum GgaA gene results in a 4196 bp
amplicon (Lanes 25, 26, 30, 31) , replacing the 1789 bp amplicon of the wild type (WT) SN15. MW, Molecular weight marker; WT, S. nodorum SN15 gDNA; NTC, no template PCR control; the remaining lanes labeled by mutant culture number. Lanes 1, 2, 11, 20, 32, 34, no observed amplification or (B) Gba1 locus of strains transformed with the Gba1 homologous
disruption construct. A AZD1390 band of 6.1 kb represents the wildtype locus and 7.6 kb the locus having undergone homologous recombination with the disruption construct. Lane 1, 1 kb ladder; Lane 2, S. nodorum SN15 (wildtype); Lanes 3–8, a representative selection of transformants. Strains represented in lanes 4, 6 and 7 have all undergone homologous recombination and represent Gba1 mutants. Figure S3. Light microscopy of the asexual spores of S. nodorum, harvested from the wild-type SN15 and mutant strains gna1-35, gba1-6 and ggaA-25. (PDF 20 VE-822 nmr MB) Additional file 2: Table S1. Sequences of primers used in this study. (DOCX 79 KB) References 1. Solomon PS, Lowe RGT, Tan KC, Waters ODC, Oliver RP: Stagonospora nodorum : cause of stagonospora nodorum blotch of wheat. Mol Plant Pathol 2006, 7:147–156.PubMedCrossRef 2. Oliver RP, Solomon PS: New developments in Gefitinib ic50 pathogenicity and virulence of necrotrophs. Curr Opin Plant Biol 2010, 13:415–419.PubMedCrossRef
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