Results: TRPV1 messenger RNA
was not induced in PBMCs of healthy controls or alcoholic liver cirrhosis patients after LPS stimulation. There was significant mature IL-1 β secretion in PBMCs of alcoholic liver cirrhosis patients when compared with healthy controls as assayed by ELISA (323.9±18.34 pg/ml N=7 vs. 14.66 ± 2.35 pg/ml N=5) and western-blot. TRPV1 antagonists (AMG9810 and A784168) attenuated NLRP3 inflammasome signaling (IL-1 β secretion) in a similar fashion to calcium chelator (BAPTA AM) or calcium signaling antagonist (2-APB). 9S-hy-droxy-10E,12Z-octadecadienoic acid (1 μM), a TRPV1 agonist, further enhanced mature IL-1 β secretion in PBMCs of alcoholic liver cirrhosis patients. Conclusion: 9(S)-HODE acts through TRPV1 to enhance NLRP3 mediated NVP-BKM120 in vitro pro-inflammatory signaling in PBMCs of alcoholic liver cirrhosis patients. Disclosures: Shirish Barve – Speaking and Teaching: Abbott Craig J. McClain – Consulting: Vertex, Gilead, Baxter, Celgene, Nestle, Danisco, Abbott, Genentech; Grant/Research Support: Ocera, Merck, Glaxo SmithKline; Speaking and Teaching: Roche The following people
have nothing to disclose: Qifa Xie, Mohammad K. Mohammad, Matthew C. Cave, Irina Kirpich Purpose: Experimental alcohol-induced liver injury is exacerbated by a high polyunsaturated fat diet rich in linoleic MLN8237 research buy acid. We postulate that bioactive oxidized linoleic acid metabolites (OXLAMs) play a critical role in the development and progression of alcohol-mediated
hepatic inflammation and injury. OXLAMs are endogenous ligands for the Transient Receptor Potential Vanilloid 1 (TRPV1). The aim of the study was to evaluate the role of signaling through TRPV1 in an experimental animal model of alcoholic liver disease (ALD). Methods: C57BL/6 wild type (WT) and Trpv1 knock out (Trpv1−/−) male mice were fed a Lieber-DeCarli diet containing 5% ethanol for 10 days, followed by a single dose of ethanol (5 g/kg body weight) by gavage (chronic-binge model). Liver steato-sis and injury were evaluated by histopathology and plasma ALT activity. Expression of genes and proteins associated with liver inflammation was analyzed. Plasma OXLAM levels were determined. In vitro studies using HepG2 cells were performed to evaluate OXLAM/TRPV1 signaling. Results: Chronic-binge alcohol selleck chemicals administration resulted in a marked increase in plasma OXLAM levels, specifically 9-HODE and 13-HODE, in parallel with up-regulation of hepatic Trpv1 in WT animals. These effects were associated with hepatic steatosis, inflammation and injury. Genetic depletion of Trpv1 did not blunt hepatic steatosis caused by EtOH, but ameliorated hepatic injury as assessed by ALT levels (354.7+54.0 U/L in WT vs 130.6+30.5 U/L in Trpv1−/−, p<0.05). Trpv1 deficiency protected from chronic-binge alcohol-induced hepatocyte death assessed by caspase-3 activity and TUNEL staining.
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