Sera from the animals displayed significantly a blocking activity in the membrane feeding assay of An. stephensi mysorensis.
Conclusions: This is the first report on P. vivax WARP expression in E. coli that provides an essential base for development of the malaria TBV against P. vivax. This may greatly assist in malaria elimination,
especially in the oriental R428 molecular weight corner of WHO Eastern Mediterranean Regional Office (WHO/EMRO) including Afghanistan, Iran and Pakistan.”
“High temperature during summer greatly affects animal production due to altered reproductive and metabolic functions. However, information regarding high throughput analysis of change in gene expression in diary animals are relatively nil. In present study, gene expression profiling by microarray was done in peripheral blood leukocytes of heat exposed (42 degrees C, 4 h) cattle (n = 3), Tharparkar (Bos indicus). A total 460 transcripts were differentially expressed with a fold change of >= 2. Randomly selected real-time validation showed that 73.08% correlation with microarray data. Functional annotation and pathway study of the DEGs reveals that, up-regulated genes significantly (P < 0.05) affect the protein processing and NOD like receptor pathways, while down regulated genes were significantly (P < 0.05) found 4-Hydroxytamoxifen to associated with Glycolytic
pathways. In conclusion, the present study showed that heat stress affects expression buy AZD1152 of significant number of genes in peripheral blood leukocytes and further analysis is required to understand their functional role in livestock. (C) 2013 Elsevier Ltd. All rights reserved.”
“Klebsiella pneumoniae is a well-known opportunistic pathogen, often causing catheter-associated urinary tract infections. Biofilm formation on the catheter surfaces is an important step in the development
of these infections. To identify the genes involved in the ability of K. pneumoniae to form a biofilm on abiotic surfaces, a novel strategy was used. A clone library was constructed by cloning the entire K. pneumoniae genome of the clinical isolate C3091 into a fosmid vector and the clone library was expressed in Escherichia coli. A total of 1152 clones were screened for enhanced biofilm formation compared with the E. coli parent strain using a biofilm microtiter plate assay. Nine clones with significantly enhanced biofilm formation were identified, subjected to random Tn5 transposon mutagenesis, screened for biofilm deficiency and the biofilm-promoting genes identified. Five of the clones contained the type 3 fimbriae gene cluster, a well-known K. pneumoniae virulence factor and biofilm promoter. Thus, the effectiveness of our approach was confirmed. Furthermore, genes encoding cell surface proteins and proteins involved in metabolism, none of them previously associated with biofilm formation in K. pneumoniae, were identified by our screening method.
No related posts.