The expression of the single mutant Y96F in an Escherichia coli host resulted in indigo formation (approximately 23 mg/l), of which productivity was comparable to the highest reported so far using other P450cam mutant cultures. On the other hand, recombinant E. coli that harbored F87W-Y96F exhibited little activity for indigo production. However, co-expression of E. coli glycerol dehydrogenase (GLD) with F87W-Y96F triggered a marked increase in the productivity of indigo (approximately 20 mg/l) without external glycerol addition. These results indicate the potential of introducing GLD-mediated NADH regeneration that utilizes endogenous NAD(+) and glycerol in E. coli to enhance markedly the catalytic performance
of a low-activity recombinant P450cam system. The present E. coil whole-cell biocatalysts might be applicable to practical selleck chemical microbial indigo production. KYT-0353 (C) 2010 Elsevier B.V. All rights reserved.”
“Purpose: This anatomic biomechanical study was undertaken to gain insight into the underlining mechanism of tipping of the maxillary segments during transverse expansion using tooth-borne and bone-borne distraction devices.
Materials and Methods: An anatomic biomechanical study was performed on 10 dentate human cadaver heads using tooth-borne and
bone-borne distraction devices.
Results: The amount of tipping of the maxillary halves was greater in the tooth-borne group, but the difference was not significant. Four of the specimens demonstrated an asymmetrical widening of the maxilla.
Conclusions: Segmental tipping was seen in both study groups. In this anatomic model, tooth-borne distraction led to greater segmental tipping compared with bone-borne distraction. Keep in mind, however, that this anatomic model by no means depicts a patient situation, and any extrapolation from it must be done with great care. The fact that the tooth-borne group demonstrated greater tipping
might reflect find more the general opinion that bone-borne distraction causes less segmental angulation than tooth-borne distraction. Some tipping was seen in the bone-borne group, suggesting that overcorrection to counteract relapse will be necessary with this treatment modality. (c) 2009 American Association of Oral and Maxillofacial Surgeons”
“Multiferroic lead lanthanum titanate-cobalt iron oxide nano-composite thin films are synthesized by sol-gel route. A percolative distribution of cobalt iron oxide phase, with percolation threshold similar to 8 vol%, is identified in these films. Large magnetoelectric coupling coefficient (similar to 250 mV/cm.Oe) is found in the composite films with cobalt iron oxide volume contents in the vicinity of its percolation threshold. The composite films with dilute magnetostrictive volume contents yield superior multiferroic properties to its counterparts with relatively higher magnetostrictive volume contents. (C) 2011 American Institute of Physics. [doi: 10.1063/1.
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