The preparation and characterization of PLA-based composites with MCC and Ag nanoparticles by twin-screw extrusion followed by injection molding is reported. A film procedure was also performed to obtain PLA and PLA composite films with a thickness ranged between 20 and 60 mu m. The analysis of disintegrability in composting conditions by means of visual, morphological, thermal, and chemical check details investigations was done to gain insights into the post-use degradation processes. Tensile test demonstrated the MCC reinforcing effect, while
a bactericidal activity of silver-based composites against a Gram-negative bacteria (Escherichia coli) and a Gram-positive bacteria (Staphylococcus aureus) was detected at any time points and temperatures analyzed. Moreover, the disintegrability in composting showed that MCC is able to promote the degradation process. The combination of MCC and Ag nanoparticles in PLA polymer matrix offers promising perspectives to realize multifunctional ternary composites SHP099 in vitro with good mechanical response and antibacterial effect, maintaining the optical transparency and the disintegrability, hence suitable for packaging applications. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012″
“It has recently been shown that alteration of the methylation pattern
of imprinted genes is associated with different types of male infertility. The objective of our study was to investigate the methylation pattern of selected gene promoters in sperm of patients with abnormal protamine replacement. The promoters of OCT4, SOX2, IPI-145 molecular weight NANOG, HOXC11, miR-17 and CREM were analyzed using bisulfite sequencing and the percentage of DNA methylation was compared between
patients with an abnormal protamine 1/protamine 2 (P1/P2) ratio and normozoospermic controls. No significant quantitative differences were found between groups of patients with either an abnormally high or low P1/P2 ratio compared to normal controls. However, two individual samples from infertile subjects (2/20, 10%) showed an altered methylation pattern for the CREM gene promoter that was not found in control samples. These two samples had a significantly higher (P < .05) promoter methylation (5.58 and 4.23%, respectively) compared to the control group (0.46%). In conclusion, in our pilot study, extreme methylations defects were not seen broadly in severely infertile men. However, two patients exhibited altered methylation of the CREM gene, which may be either causative or a result of abnormal protmaine replacement. Asian Journal of Andrology (2011) 13, 342-346; doi:10.1038/aja.2010.160; published online 3 January 2011″
“The Pd doped amorphous carbon (a-C:Pd) films were deposited on n-Si substrates with a native SiO(2) layer using direct current magnetron sputtering. The light-induced spatial sensitivity of the resistance of the a-C:Pd/SiO(2)/Si structure was investigated.
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