coli ATCC25922 and low production of NDM-1 [27]. False positive results of carbapenemase production by the MHT among isolates with resistance or reduced susceptibility to carbapenem result from low-level carbapenem hydrolysis by CTX-M type ESBLs and ESBL production coupled with porin loss [28, 29]. These data mentioned above indicated that the detection of carbapenemases by the MHT was challenged, especially the detection of NDM-1. NDM-1 was mainly found in Enterobacteriaceae in south Asia, Europe and America [5, 6, 30]. In contrast, it was initially and mainly described in Actinetobacter spp. clinical isolates in China [11–14], even emergence of dissemination
of NDM-1-producing A. pittii (27 isolates) in an intensive care unit [31]. Recently, a higher isolation of NDM-1-producing A. baumannii from the sewage of the hospitals in Beijing, the capital of China, was described, indicating that the hospital sewage may be one of the diffusion reservoirs Selleck Barasertib of NDM-1 producing bacteria [32]. However, one screening effort revealed no bla NDM-1 expression among 3439 E. coli and 2840 K. pneumoniae isolates from 57 hospitals
representing 18 provinces in China [11]. Recently, bla NDM-1 began to emerge in Enterobacteriaceae from China [15, 16, 33]. Two clonally unrelated K. pneumoniae isolates from two teaching hospitals in Nanchang, central China, were found to harbor bla NDM-1[16]. Coexistence of bla NDM-1 and Ro 61-8048 in vitro bla IMP-26 was identified among a carbapenem-resistant Enterobacter MM-102 mw cloacae clinical isolate from southwest China [33]. Sporadic emergence of bla NDM-1 in E. coli clinical isolates in the present study further corroborates the evidence that bla NDM-1 carriage
extends beyond Actinetobacter spp into Enterobacteriaceae in China. Another study from China also found that a E. coli clinical isolate isolated from the ulcer secretion of patient with diabetes-related foot complications harbored bla NDM-1[15]. International travelers to the Indian subcontinent, are prone to acquire the Protein kinase N1 infections caused by NDM-1-producing organisms [4, 5]. However, the two patients harboring NDM-1-producing E. coli had never traveled to outside China. Antimicrobial susceptibility profiling The results of antimicrobial susceptibility of E. coli WZ33 and WZ51 are listed in Table 1. Both tested isolates were multi-resistant to clinically frequently used antimicrobials, including ampicillin, piperacillin, piperacillin/tazobatam, cefotaxime, ceftazidime, cefepime, cefoxitin, aztreonam, imipenem, meropenem, ertapenem and gentamicin, levofloxacin, but susceptible to trimethoprim/sulfamethoxazole, amikacin, fosfomycin, tigecycline and polymyxin B. Most of NDM-1-producing isolates were highly resistant to clinically available antibiotics except to tigecycline and colistin [4]. Table 1 MIC values of antimicrobials for E.coli isolates carrying blaNDM-1 and their transformants Antimicrobials MIC values (μg/ml) E. coli WZ33 a E.
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