Protein matches with significant (p < 0.05) Mowse Scores and ≥ 2 matching peptides were regarded as possible candidates for identification. 2) Annotation of uncharacterised proteins was based on sequence homology to characterised Swiss-Prot proteins using BlastP. Proteins were given a full annotation if they had > 80% sequence identity to www.selleckchem.com/products/a-1210477.html a characterised Swiss-Prot protein or a putative annotation if they had 50-80% sequence identity to a characterised protein. Remaining proteins were assigned a “”predicted”" function if InterPro domains were predicted using
InterProScan. 3) Observed mass on reference gel calibrated with molecular weight standards (14.4-97.4 kDa). 4) The spot is most likely a fragment as the retrieved peptides were localized in one of the ends of the protein sequence. 5) Mass above or below calibration range 6) The protein is predicted to contain MCC950 in vivo a signal peptide. 7) The protein is predicted to be glycosylated. Table 4 Identified proteins with lower levels on medium with starch + lactate Protein Spot Identification1 Expression Annotation 2 Id. Mass kDa 3 Database Acc. no. Mass kDa pI MP Score SC % Cl. no. Profile Aldehyde dehydrogenase 6605 53 Swis-Prot P41751 54 6.0 10 908
34 37 Aldehyde dehydrogenase 6615 52 Swis-Prot P41751 54 6.0 7 646 20 38 Beta-glucosidase 1 precurser 6360 1305 HDAC inhibitor NCBInr Q30BH9 94 4.7 5 267 6 36 Fructose-biphosphate aldolase 6766 39 NCBInr A2QDL0 40 5.5 8 697 28 37 Predicted estherase/lipase/thioesterase 6451 82 NCBInr A2QTP5 84 5.4 9 543 18 37 Predicted fumaryl-acetoacetate hydrolase 6663 47 NCBInr A2QIN6 45 5.2 6 611 24 38 Predicted
glutathione-S-transferase 6952 27 NCBInr A2R874 24 5.1 5 391 31 37 Predicted NAD-dependant epimerase/dehydratase 6707 43 NCBInr A2R992 38 5.7 7 397 26 38 Predicted ribose/galactose isomerase 7035 18 NCBInr A2QCB3 17 7.7 7 593 61 36 Predicted Zn-containing alcohol dehydrogenase 6718 42 NCBInr A2QAN5 39 5.8 4 298 19 38 Putative 1-aminocyclopropane-1-carboxylate deaminase 6715 42 NCBInr Cross sp. Q7S3B7 39 5.8 2 115 11 38 Putative glutamate carboxypeptidase-like 6609 53 NCBInr A2QY36 53 5.2 12 811 29 38 Putative HIT family protein 1 7091 135 NCBInr PD184352 (CI-1040) A2QLN7 15 6.3 3 227 40 37 Putative H-transporting two sec tor ATPase subunit F, vacuolar 7083 14 NCBInr A2QCE6 14 5.3 4 340 44 37 Putative NADH ubiquinone reductase, 40 kDa subunit, mitochondrial 6738 41 NCBInr A2QSH0 43 6.7 5 307 17 38 Putative peroxiredoxin pmp20, peroxisomal membrane 7031 18 NCBInr A2R6R3 18 5.6 5 431 37 38 Superoxide dismutase Cu-Zn, cytoplasmic 7046 17 Swiss-Prot A2QMY6 16 5.9 5 323 38 36 Ubiquitin-like protein 7113 115 NCBInr A2QKN1 9 5.8 5 272 60 37 Uncharacterised protein 7002 21 NCBInr A2QLX7 20 6.1 7 592 55 8 Uncharacterised protein 7074 154 NCBInr A2QBG0 34 5.1 6 609 24 38 See legend and notes to table 3.
No related posts.