Together, iTRAQ analysis suggests that MucE signaling affected both AlgU-dependent and AlgU-independent protein expression. Conclusions The alternative sigma factor AlgU was responsible for mucE transcription. Together, our results suggest there is a positive feedback regulation of MucE by AlgU in P. aeruginosa, and the expression of mucE can be induced by exposure to certain cell wall stress agents, suggesting that mucE may be part of the signal transduction that MX69 in vitro senses the cell wall stress to P. aeruginosa. Acknowledgements This work was supported by the National Aeronautics and Space Administration West Virginia Space Grant Consortium (NASA WVSGC)

and the Cystic Fibrosis Foundation (CFF-YU11G0). F.H.D. was supported by grants from the NASA Graduate Student Researchers Program (NNX06AH20H), NASA West Virginia Space Grant Consortium, and a post-doctoral fellowship from the Cystic Fibrosis

Foundation check details (DAMRON10F0). T.R.W. was supported through the NASA WVSGC Graduate Research Fellowship. H.D.Y. was supported by NIH P20RR016477 and P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence. Electronic supplementary material Additional file 1: Supplementary materials and methods. (DOC 782 KB) References 1. Govan JR, Deretic V: Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia . Microbiol Rev 1996,60(3):539–574.PubMed 2. May TB, Shinabarger D, Maharaj R, Kato J, Chu L, DeVault JD, Roychoudhury S, Zielinski NA, Berry A, Rothmel RK, et al.: Alginate HDAC activation synthesis by Pseudomonas aeruginosa : a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin Microbiol

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